Stopping the Biological Clock

Author: Bradford Kolb, M.D., F.A.C.O.G.,

Oocyte Crypreservation- Stopping The Biological Clock by Bradford Kolb, M.D., F.A.C.O.G., Board Certified, Reproductive Endocrinology and Infertility and Christy Jones, CEO, Extend Fertility

For decades, sperm and embryos (fertilized eggs) have been successfully frozen for the purposes of fertility preservation and donation for men and couples. Although both sperm and embryo cryopreservation have become commonplace, the freezing of unfertilized oocytes (or eggs) for similar applications in women has not historically delivered the acceptable success rates necessary to drive adoption across the board.

Unlike sperm and embryos, oocytes did not survive the freeze/thaw process well, primarily because the egg is the largest cell in the human body and comprised mostly of water. The water inside the cell forms ice crystals that destroy the egg during the traditional freezing process. Prior to 2002, the success rate of live births from frozen eggs was 1-3% globally, with few babies born from frozen eggs over decades of attempts.

Fortunately, a number of advances in our knowledge of oocyte physiology and laboratory techniques are rapidly changing this dream into a reality.

The ability to preserve unfertilized oocytes is profound if one looks at the potential benefits and some of the controversies surrounding reproductive medicine. Egg-freezing should appeal to a broad range of women. Ultimately, the common factors that link all of these women are the strong desire to have a family and the willingness to take proactive steps to give themselves the best odds possible.

1. While some couples are comfortable with the concept of embryo freezing, many have moral and ethical dilemmas regarding this issue. For those that believe that life is created at the moment of conception, each frozen embryo represents a life and, if unused, a life unfulfilled. Oocyte cryopreservation, like sperm cryopreservation, presents us with the possible opportunity to preserve one's fertility while avoiding these ethical dilemmas.

2. Oocyte cryopreservation provides young women facing chemotherapy or irradiation for treatment of life-threatening disease, such as cancer, the opportunity to preserve their
fertility. The importance of this cannot be understated. Treatment regimens for many of the malignancies faced by adolescents and young adults result in the destruction of their gametes (sperm or eggs). The advancements in cancer treatments are also ensuring that many of these cancer survivors are living long, productive lives. Thus, the opportunity to preserve their ability to have children later in life is critical.

3. The professional and personal opportunities for women have exploded over the past 30 years, encouraging many women to postpone motherhood. Unfortunately, the biology of female eggs hasn't kept pace and women often face challenges starting their families later in life. Women, who are born with a limited supply of eggs, start to experience diminished fertility rates in the late twenties and this rapidly accelerates as they reach their mid-thirties. Childlessness is one of the biggest concerns for professional women. Studies show that the majority of the 33% of high-achieving women that are childless at ages 41-55 did not choose to be childless. In fact, more than a quarter of high-achieving women in the 41-55 year old age group said they would still like to have children. For women in this category, the ability to preserve their eggs (and thus their future fertility) gives them more flexibility as to when they can start their families.

4. Oocyte cryopreservation will allow those who need to resort to egg donation (the use of someone else's eggs due to the diminished fertility potential of their own eggs) more
affordable treatment options. Today, when a couple chooses to use donor eggs, they must bear the cost of the entire donor's IVF cycle alone. The ability to use only the limited number of eggs necessary while freezing the unused eggs will allow couples using donor's eggs to cut their expenses dramatically. This will allow individuals who were unable to afford such services the opportunity to pursue having children.

Techniques Given the magnitude of the need, clinicians around the world have raced to develop a technique for successful egg-freezing, and beginning in 2002, promising results ranging from 20-40% successful pregnancy rate (on par with a woman's natural peak fertility rate) were published. The key difference over previous techniques was the change in cryoprotectants used to protect the egg during the freezing process. Cryoprotectant acts as an "antifreeze" to protect the delicate egg as the temperature drops.

Before attempting to understand how our ability to cryopreserve unfertilized eggs has been achieved, it is important to understand how sperm and embryo (fertilized oocytes) cryopreservation has long been commonplace. The major problem faced in freezing a cell is to minimize damage to the membranes induced by ice crystal formation. Intracellular ice formation is dangerous because it may rupture the cell membranes causing cellular destruction. The smaller the cell, the less likely ice crystal formation will occur.

Sperm cells are about 180th the size of a mature egg and thus can be easily preserved. Embryos, which are eggs that have been fertilized, are approximately the same size as mature eggs, but are much more likely to survive the freezing/thawing cycles.

This is due to the fact that the eggs' membranes undergo dramatic changes during fertilization, making them more likely to tolerate the stresses associated with freezing. The use of cyroprotectants and highly controlled freezing/thawing rates have dramatically improved the survival rates of frozen sperm and frozen embryos. In many ways, the lessons learned from freezing sperm and embryos are being applied to freezing oocytes. However, the unique nature of the female egg has required additional study and technological developments.

A number of approaches have been taken in order to maximize the survival rates of frozen oocytes. The greatest success has been achieved with protocols that use slow freezing/rapid thaw protocols. Critical to any freezing protocol is the use of cryoprotectants.

Cryoprotectants act by a variety of means to reduce the amount of water that crystallizes within the cell and protects the cell during the freezing process. Common cryoprotectants include an alcohol (1,2-propranediol), a carbohydrate (sucrose) and a solvent (DMSO). The concentration and the duration of exposure to most cryoprotectants (alcohols and solvents) are critical, as exposure to high concentrations or exposure for prolonged periods of time can result in damage to the cell. We have found that increasing the concentration of sucrose (a relatively safe cryoprotectant that works by pulling water out of the cell) results in significantly improved survival rates, fertilization rates and pregnancy rates for frozen oocytes.

We also have found that the removal of the cryoprotectant with progressive dilution is a critical step in the thawing process. If oocytes are placed directly in a medium without cryoprotectant after thawing, they can swell and burst. The use of nonpermeating molecules (molecules that do not enter in the thawing cell) such as sucrose, act to oppose the inflow of water into the cell and thus prevent the membrane from bursting.

Others have recently undertaken investigations using a process called vitrification; a process that utilizes ultrarapid freezing techniques. While some pregnancies have been achieved utilizing this technique, it has not been shown to be more efficacious than slow freeze/rapid thaw protocols and is more susceptible to human error.

This process exposes the egg to potentially damaging levels of cryoprotectant and direct exposure to liquid nitrogen. Exposure to liquid nitrogen is a critical factor in this age of concern over infectious agents. The infectious agents, while rare, can result in life-threatening illnesses. This is a critical concern as the cryopreserved cells are stored in common tanks and a single tank may contain thousands of cells.

Regardless of the freezing technique, the oocyte goes through a number of changes that make it less likely to fertilize using standard co-incubation techniques (the mixing of eggs and sperm together).

The understanding that the zona pellucida (an exoskeleton that covers the outside of the egg) undergoes changes due to the premature release of the cortical granules (these are normally released at the time of fertilization and prevent multiple sperm from fertilizing the egg) is an important factor that has lead to improved success with frozen eggs.

This has led to changes in how frozen oocytes are fertilized. With the introduction of intracytoplasmic sperm injection (ICSI), the results for fertilization, embryo development, and for implantation rates (attachment of the embryo to the uterus) are approaching those obtained with fresh embryos.

Conclusions

We are just reaching a time where it is becoming feasible to preserve unfertilized, mature oocytes. These are harvested after taking fertility medications to induce the maturation of a number of oocytes. Women of this generation want more options and power when it comes making life decisions and plans. This is an exciting next step in the long line of developments in the field of women's reproductive health - on par with the introduction of the birth control pill.

Dr. Bradford Kolb is a reproductive endocrinologist and OB/GYN at Huntington Reproductive Center in Pasadena, CA Phone: (626) 440-9161 or Toll Free (866) HRC-4IVF Website: http://www.havingbabies.com

Christy Jones is the CEO and founder of Extend Fertility in Boston, MA Phone: (800) 841-7197 Email: info@extendfertility.com Website: http://www.extendfertility.com

REFERENCES:
U.S. Census Bureau. "Distribution of Women by Average Number of Children Ever Born, by Race, Age, and Marital Status." Fertility of American Women Current Population Survey (June 2000).

National Parenting Association. "Groundbreaking Study Exposes A Crisis Among Successful Women: The Survey Behind Sylvia Ann Hewlett's 'Creating a Life.'" National Parenting Association Web site.



About the author:
Bradford Kolb, MD, F.A.C.O.G.

Undergraduate -University of California, Irvine Medical School
University of California, Irvine

Residency Obstetrics & Gynecology-Northwestern University

Fellowship Reproductive Endocrinology & Infertility-University
of Southern California

Board Certifications-Reproductive Endocrinology and Infertility
Obstetrics and Gynecology
http://www.havingbabies.com/staff_kolb.html